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ZFIN ID: ZDB-FIG-101122-37
Nowak et al., 2005 - Perp is required for tissue-specific cell survival during zebrafish development. Cell death and differentiation   12(1):52-64 Full text @ Cell Death Differ.
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 8 Loss of Perp causes caspase-dependent apoptosis. Acridine orange (a–f) and FITC-VAD-fmk (h,i) stainings of live embryos at 30 hpf; (a,c,e,h) injected with perp MO, (b) injected with perp MO and treated with caspase inhibitor zVAD-fmk, (c,f,i) injected with perp 4 mm MO, (d) uninjected control; (a–d,h,i) lateral view on tip of tail; (e,f) magnified lateral view on notochord. Note that in the notochord of perp morphants (e), all cells display altered, roundish shapes, while only some of the cells have become acridine orange positive. (g) Diagram illustrating average numbers of acridine orange-positive cells in embryos as shown in (a–d) within the tail region posterior of the anus. Number of evaluated embryos: control n=5; perp 4 mm MO-injected n=5; perp MO-injected n=6; perp 4 mm MO-injected+zVAD-FMK n=8

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-perp standard conditions Prim-15 integument apoptotic, abnormal
Prim-15 integument epithelial cell circular, abnormal
Prim-15 notochord apoptotic, abnormal
Prim-15 ventral fin fold cell circular, abnormal
ZFIN wishes to thank the journal Cell death and differentiation for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
ZFIN also wishes to thank Matthias Hammerschmidt for permission to reproduce figures from this article.
Full text @ Cell Death Differ.