ZFIN ID: ZDB-FIG-100831-3
Chi et al., 2010 - Cardiac conduction is required to preserve cardiac chamber morphology. Proceedings of the National Academy of Sciences of the United States of America   107(33):14662-14667 Full text @ Proc. Natl. Acad. Sci. USA
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. S3 cx46 Expression and morpholino knockdown confirms that cx46 is the affected gene in dco mutants. (A–D) Whole-mount RNA in situ hybridization reveals cx46 expression in the heart and lens. Expression of cx46 at (A) 36, (B) 42, (C) 48, and (D) 72 hpf. A, atrium; V, ventricle. (E) cx46 and β-Actin control RT-PCR from FACS-sorted myocardial and endocardial cells at 36, 48, and 72 hpf. (F) Morpholino knockdown of cx46 recapitulates dco cardiac phenotype. Of WT embryos injected with an ATG cx46 morpholino, 88% are indistinguishable from dco mutant embryos and display severe impairment of cardiac conduction (n = 125/142).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gja3 WT standard conditions Prim-25 heart tube ISH
Prim-25 to Protruding-mouth Not Detected endocardium RTPCR
Prim-25 to Protruding-mouth myocardium RTPCR
High-pec heart ISH
Long-pec atrioventricular canal ISH
Long-pec cardiac ventricle ISH
Protruding-mouth atrioventricular canal ISH
Protruding-mouth cardiac ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gja3 standard conditions Long-pec cardiac conduction system development process quality, abnormal
Long-pec heart contraction asynchronous, abnormal
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA