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ZFIN ID: ZDB-FIG-100816-26
Rhodes et al., 2010 - Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved. Developmental Biology   344(2):637-649 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 5 Reduced myca expression in rad21nz171 mutants and smc3 morphants. A, B, expression pattern of myca in whole-mount wild type and rad21nz171 embryos at 24 h.p.f. and 36 h.p.f. respectively (anterior to the left). myca expression (purple) in the brain and eye of wild type is absent in rad21nz171 embryos. C, D, Expression of myca is also greatly reduced in smc3 morphants at 24 h.p.f. and 36 h.p.f. respectively (anterior to the left). Embryos were injected with antisense morpholino oligonucleotides targeting the start codon (smc3ATG-MO) or the 3′ donor site of exon 1 (smc3Spl1-MO) of the smc3 gene to create two smc3 morphants. E, F, The expression of myca in smc3 morphants (smc3ATG-MO and smc3Spl-MO) is significantly reduced compared to wild type embryos as measured by quantitative PCR at 24 h.p.f. and 36 h.p.f. respectively.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myca WT standard conditions Prim-5 brain ISH
Prim-5 eye ISH
Prim-25 brain ISH
Prim-25 eye ISH
WT + MO4-smc3 standard conditions Prim-5 brain ISH
Prim-5 eye ISH
Prim-25 brain ISH
Prim-25 eye ISH
WT + MO5-smc3 standard conditions Prim-5 brain ISH
Prim-5 eye ISH
Prim-25 brain ISH
Prim-25 eye ISH
rad21anz171/nz171 standard conditions Prim-5 Not Detected brain ISH
Prim-5 Not Detected eye ISH
Prim-25 Not Detected brain ISH
Prim-25 Not Detected eye ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 344(2), Rhodes, J.M., Bentley, F.K., Print, C.G., Dorsett, D., Misulovin, Z., Dickinson, E.J., Crosier, K.E., Crosier, P.S., and Horsfield, J.A., Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved, 637-649, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.