ZFIN ID: ZDB-FIG-100525-9
Sarmah et al., 2010 - Sec24D-dependent transport of extracellular matrix proteins is required for zebrafish skeletal morphogenesis. PLoS One   5(4):e10367 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 8 Knockdown of sec24c, and combined loss of sec24d and sec24c phenotypes.

Analysis of wild types (A, E, I), sec24c-UTR-MO (B,F,J), bulldog (C, G, K) and double sec24c-UTR-MO+ sec24d-UTR-MO (D, H, L) injected embryos. (A–D) Images of live wild types (A), sec24c-UTR-MO, (2.0 ng injected, B), bulldog (C) and sec24c-UTR-MO+ sec24d-UTR-MO (2.0 ng each, D). The sec24c morphants have reduced body length (blue arrows), but the head appears normal. Double morphants for sec24d+sec24c are significantly shorter than bulldog larvae, almost completely lack fin-folds and have reduced jaw region (red arrow) and pronounced heart edema (D). (E–H) Alcian blue preparations of the head skeleton. The Alcian blue staining confirms normal craniofacial cartilages in sec24c morphants. (I–L) Histological sections counter-stained with toluidine blue at 4 dpf. Insets indicate the plane of the sections. (I,J) Histological, sagittal plastic sections stained with toluidine blue show normal jaw opening and surrounding cartilages in wild types (I) and sec24c morphants (J). Purple staining patterns for glycosaminoglycans of the ECM are also comparable. In contrast, the pharyngeal skeleton of sec24d+sec24c double mutants fails to stain with Alcian blue (H). Histological sections reveal patterned arches that are largely devoid of metachromatically stained cells (L). Abbreviations: cb, ceratobranchial; ch, ceratohyal; ep, ethmoid plate, m, Meckel′s cartilage.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-sec24d + MO2-sec24c standard conditions Day 4 auditory capsule malformed, abnormal
Day 4 chondrocyte differentiation arrested, abnormal
Day 4 embryonic viscerocranium morphogenesis process quality, abnormal
Day 4 extracellular matrix constituent secretion decreased occurrence, abnormal
Day 4 pectoral fin cartilage malformed, abnormal
Day 4 pharyngeal arch cartilage extracellular matrix decreased volume, abnormal
AB + MO2-sec24c standard conditions Day 4 pharyngeal arch cartilage physical object quality, normal
Day 4 whole organism decreased length, abnormal
sec24dm606/m606 (AB) standard conditions Day 4 embryonic viscerocranium morphogenesis process quality, abnormal
Day 4 pharyngeal arch cartilage physical object quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One