Fig. 3

lta4h MO Knockdown Results in Increased Susceptibility to Mm

(A) Representative fluorescence images of control (top) and lta4h morphant (bottom) fish 5 dpi with 173 bacteria. The scale bar represents 200 μm.

(B) Mean bacterial loads per embryo for control (wt) and lta4h morphant embryos at 1.5 and 6 dpi with 150 bacteria (n = 4 groups of 5 animals for each time point). Error bars represent the SD.

(C) Survival of control and lta4h morphant fish mock-injected or injected with 177 bacteria (n = 25 per group). Hazard Ratio for death of infected morphants = 9.0, p < 0.0001 (Kaplan Meier method with log-rank [Mantel-Cox] test). Data representative of three independent experiments.

(D) Fluorescence image of non-cording bacteria within a granuloma in control animals (top) and cording bacteria in lta4h morphant (bottom) at 5 dpi with infection dose of 150 bacteria. The scale bar represents 20 μm.

(E) Mean proportion of animals with cording in three independent groups of 15–40 animals 4 dpi with 173 bacteria. p = 0.003 (Student′s unpaired t test). Error bars represent the standard error of the mean (SEM).

(F) Bacterial burden at 5 dpi as determined by fluorescence pixel counts (FPC) for vehicle-treated or 100 μM bestatin-treated animals after injection with ∼150 CFU Mm. p = 0.03 (Student′s unpaired t test).

(G) Fluorescence image of non-cording bacteria within a granuloma in control animals (top) and cording bacteria in animals treated with 100 μM bestatin (bottom) at 5 dpi as described in (F). The scale bar represents 10 μm.

(H) Quantitation of cording at 4 dpi in five independent groups of 10–30 animals treated with vehicle or 100 μM bestatin after injection with 100–200 CFU Mm. p = 0.007 (Student′s unpaired t test). Error bars represent the SEM.