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ZFIN ID: ZDB-FIG-090817-40
Huang et al., 2009 - Collagen IX is Required for the Integrity of Collagen II Fibrils and the Regulation of Vascular Plexus Formation in Zebrafish Caudal Fins. Developmental Biology   332(2):360-370 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Days 14-20
PHENOTYPE:
Fish:
Observed In:
Stage: Days 14-20

Fig. 7 Vascular plexus causes wavy and fused fin rays in prp fish. (A–C) Blood vessel development in the caudal fin of wild-type (WT) fish. (D–F) Immunostaining of osteoblasts (red) with the ZnS5 monoclonal antibody of the same fins revealed that osteoblasts developed and were patterned after blood vessels as evidenced by areas where only blood vessels were present (arrows in panels D and E). (G) A prp fin showing the vascular plexus. (H) The same prp fin stained with ZnS5 (red) showing that the osteoblast pattern was closely associated with the blood vessel pattern. (I) High magnification of a representative area of (H) showing wavy and fused osteoblast rays along with the misconnected blood vessels (arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP col22a1j131/j131; y1Tg/y1Tg standard conditions Days 14-20 caudal fin vasculature IHC
Days 14-20 post-vent vasculature IHC
y1Tg standard conditions Days 14-20 caudal fin vasculature IHC
Days 14-20 post-vent vasculature IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zns-5 IHC col22a1j131/j131; y1Tg/y1Tg standard conditions Days 14-20 caudal fin osteoblast
IHC y1Tg standard conditions Days 14-20 caudal fin osteoblast
Phenotype Details
Fish Conditions Stage Phenotype
col22a1j131/j131; y1Tg/y1Tg standard conditions Days 14-20 caudal fin interray vessel disorganized, abnormal
Days 14-20 caudal fin morphogenesis disrupted, abnormal
Days 14-20 caudal fin osteoblast mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 332(2), Huang, C.C., Wang, T.C., Lin, B.H., Wang, Y.W., Johnson, S.L., and Yu, J., Collagen IX is Required for the Integrity of Collagen II Fibrils and the Regulation of Vascular Plexus Formation in Zebrafish Caudal Fins, 360-370, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.