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ZIRC
ZFIN ID: ZDB-FIG-081218-13
Wang et al., 2008 - Initiation and propagation of posterior to anterior (PA) waves in zebrafish left-right development. Developmental dynamics : an official publication of the American Association of Anatomists   237(12):3640-3647 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: 10-13 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 10-13 somites

Fig. 3 Distinct bilateral phenotypes in cas, cha, and lft1 morphants. A: Standard analysis of lateral plate mesoderm (LPM) spaw expression categorized as left-side expression (Red), bilateral expression (green), absent expression (blue), and reversed expression (yellow). B-D: Bilateral spaw expression in LPM is categorized as (B) the left side boundary more anterior than that of the right side (left side leading); (C) both sides with equal anterior boundaries (equivalent); (D) right side boundary was more anterior than that of the left side (right side leading). Green arrowhead: leading side spaw expression boundary. E: Quantification of cas, cha and lft1 morphants with bilateral phenotype. F-H: lft1 morphants retain LR bias at 9 to 10 somite stage (SS): (F) spaw expression in the left LPM (n = 28); (G) spaw expression in the right LPM (n = 5); (H) spaw was not expressed in WT (n = 86/88) at this stage. Green arrow: spaw expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
spaw WT standard conditions 10-13 somites Not Detected lateral plate mesoderm ISH
14-19 somites lateral plate mesoderm ISH
WT + MO1-dand5 standard conditions 10-13 somites lateral plate mesoderm ISH
14-19 somites lateral plate mesoderm ISH
WT + MO2-lft1 standard conditions 10-13 somites lateral plate mesoderm ISH
14-19 somites lateral plate mesoderm ISH
WT + MO2-sox32 standard conditions 10-13 somites lateral plate mesoderm ISH
14-19 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dand5 standard conditions 10-13 somites determination of left/right symmetry disrupted, abnormal
WT + MO2-lft1 standard conditions 10-13 somites determination of left/right symmetry disrupted, abnormal
WT + MO2-sox32 standard conditions 10-13 somites determination of left/right symmetry disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.