ZFIN ID: ZDB-FIG-080918-34
Alexander et al., 1999 - casanova plays an early and essential role in endoderm formation in zebrafish. Developmental Biology   215(2):343-357 Full text @ Dev. Biol.
Anatomical Terms:
Stage: Long-pec
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1 cas mutants lack a gut tube. Nomarski optical images (A–D) and histological sections (E, F) of embryos at 24 (A, B), 36 (C, D), and 48 hpf (E, F). Compared to a wild-type sibling (A), the cas mutant (B) shows pericardial edema (arrowhead), collapsed brain ventricles (asterisks), and a thicker yolk extension (arrow). In wild-type embryos (C) the still-forming intestine (arrow) and anal opening (black arrowhead) are visible, immediately anterior to the pronephric ducts (white arrowhead). Neither the intestine nor the anal opening is evident in cas mutants (D); arrowhead indicates the cyst present in cas mutants. (E) The shh-expressing gut tube (arrow) sits between the notochord (arrowhead) and the yolk in wild-type embryos. In cas mutants (F) no gut tube or shh-expressing endodermal cells are evident and the notochord (arrowhead) rests nearly upon the yolk. Expression of shh in the floor plate is evident in both wild-type and cas mutant embryos. (A–D) Lateral views, anterior to the left and dorsal to the top; (E, F) transverse sections through the upper trunk and yolk ball.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
shha WT standard conditions Long-pec floor plate ISH
Long-pec gut endodermal cell ISH
sox32ta56/ta56 standard conditions Long-pec floor plate ISH
Long-pec Not Detected gut endodermal cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sox32ta56/ta56 standard conditions Prim-5 extension increased thickness, abnormal
Prim-5 fourth ventricle collapsed, abnormal
Prim-5 pericardium edematous, abnormal
Prim-5 tectal ventricle collapsed, abnormal
Prim-25 intestine absent, abnormal
Prim-25 proctodeum absent, abnormal
Prim-25 pronephric duct absent, abnormal
Long-pec endoderm absent, abnormal
Long-pec endoderm formation disrupted, abnormal
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Reprinted from Developmental Biology, 215(2), Alexander, J., Rothenberg, M., Henry, G.L., and Stainier, D.Y.R., casanova plays an early and essential role in endoderm formation in zebrafish, 343-357, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.