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ZFIN ID: ZDB-FIG-080917-873
Piotrowski et al., 2000 - The endoderm plays an important role in patterning the segmented pharyngeal region in zebrafish (Danio rerio). Developmental Biology   225(2):339-356 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 13 Whole-mount in situ hybridization experiments with genes that are expressed in the mesoderm. (A and B) Lateral views of two jaw muscles, the levator arcus palatini (LAP) and the dilator operculi (DO), that express Engrailed during development. (B) In 52-hpf vgo embryos these two muscles are absent, as revealed by antibody labeling with 4D9, which recognizes Eng. All other voluntary pharyngeal muscles express myoD. (C and D) Ventral views of myoD expression in 52-hpf wild-type (C) and vgo (D) embryos. In vgo embryos the muscles which are associated with the pharyngeal arches 4–7 do not express myoD or are absent.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT standard conditions Long-pec pharyngeal musculature ISH
Long-pec ventral transverse ISH
tbx1tm208/tm208 standard conditions Long-pec pharyngeal musculature ISH
Long-pec Not Detected ventral transverse ISH
tbx1tu285/tu285 standard conditions Long-pec pharyngeal musculature ISH
Long-pec Not Detected ventral transverse ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT standard conditions Long-pec dilatator operculi
IHC Long-pec levator arcus palatini
IHC tbx1tm208/tm208 standard conditions Long-pec Not Detected dilatator operculi
IHC Long-pec Not Detected levator arcus palatini
IHC tbx1tu285/tu285 standard conditions Long-pec Not Detected dilatator operculi
IHC Long-pec Not Detected levator arcus palatini
Phenotype Details
Fish Conditions Stage Phenotype
tbx1tm208/tm208 standard conditions Long-pec dilatator operculi aplastic, abnormal
Long-pec levator arcus palatini aplastic, abnormal
Long-pec ventral transverse aplastic, abnormal
tbx1tu285/tu285 standard conditions Long-pec dilatator operculi aplastic, abnormal
Long-pec levator arcus palatini aplastic, abnormal
Long-pec ventral transverse aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 225(2), Piotrowski, T. and Nüsslein-Volhard, C., The endoderm plays an important role in patterning the segmented pharyngeal region in zebrafish (Danio rerio), 339-356, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.