FIGURE

Fig. 3

ID

ZDB-FIG-080908-8

Publication

Clay et al., 2008 - Tumor necrosis factor signaling mediates resistance to mycobacteria by inhibiting bacterial growth and macrophage death

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Fig. 3

Growth of Erp Mutant Bacteria Is Partially Restored in TR1 Morphant Embryos

(A) Representative fluorescence images for control (Con) and TR1 morphant fish infected with 55 ± 7 CFU of Erp mutant (Erp) M. marinum at 4 dpi. The scale bar represents 500 μm.

(B–D) A total of 50 individual macrophages were scored for multiplicity of infection (MOI) as either containing (B) 10 or less bacteria or (C) more than 10 bacteria at 3 dpi during infection with 55 ± 7 CFU of Erp mutant bacteria. The scale bar represents 25 μm. As shown in (D), the percentage of macrophages (out of 50) scored as either having more than ten, or ten or less bacteria per macrophage ± standard deviation of the mean in TR1 morphant versus control embryos. TR1 morphants had a significantly greater percentage of macrophages with more than ten bacteria as compared to controls (p < 0.05, Student's unpaired t test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK + MO1-tnfrsf1a + MO2-tnfrsf1a
Condition:	bacterial treatment: Mycobacterium marinum
Knockdown Reagents:	MO1-tnfrsf1a MO2-tnfrsf1a
Observed In:	defense response to bacterium
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Immunity, 29(2), Clay, H., Volkman, H.E., and Ramakrishnan, L., Tumor necrosis factor signaling mediates resistance to mycobacteria by inhibiting bacterial growth and macrophage death, 283-294, Copyright (2008) with permission from Elsevier. Full text @ Immunity