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ZFIN ID: ZDB-FIG-080417-18
Blum et al., 2008 - Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo. Developmental Biology   316(2):312-322 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Prim-25

Fig. 4 Dynamic expression of junctional proteins during ISV formation. Confocal projections of fli1:EGFP embryos labelled with anti-ZO-1 (A–D) or CDH5 (E–H) antibodies and of flk1:EGFPnls embryos labelled with CDH5 (I–L) antibody at different developmental stages. (A, E, I) 22–24 hpf, (B, F, J) 26–28 hpf, (C, G, K) 30 hpf, (D, H, L) 36 hpf. Already at early stages (A, E) both proteins are localized along the stalk of the ISVs, presumably between putative tip cells on the one hand, and between stalk cells on the other hand. Spots and lines (arrows in panels C, F and J) of ZO-1 and CDH5 are visible when tip cells start to extend in anterior and posterior direction to eventually form the DLAV. Arrowheads point to parallel junctions running along the axis of the ISV. Abbreviations: see Fig. 1, nt: neural tube. Scalebars: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 Ab1-cdh5 y1Tg standard conditions 26+ somites dorsal aorta bicellular tight junction IHC
Ab1-cdh5 Prim-5 dorsal aorta bicellular tight junction IHC
Ab1-cdh5 Prim-5 intersegmental vessel bicellular tight junction IHC
Ab1-cdh5 Prim-15 dorsal aorta bicellular tight junction IHC
Ab1-cdh5 Prim-15 intersegmental vessel bicellular tight junction IHC
Ab1-cdh5 Prim-25 dorsal aorta bicellular tight junction IHC
Ab1-cdh5 Prim-25 intersegmental vessel bicellular tight junction IHC
EGFP ubs1Tg standard conditions 26+ somites dorsal aorta IFL
26+ somites epithelial cell nucleus IFL
Prim-5 dorsal aorta IFL
Prim-5 epithelial cell nucleus IFL
Prim-5 intersegmental vessel IFL
Prim-15 dorsal aorta IFL
Prim-15 epithelial cell nucleus IFL
Prim-15 intersegmental vessel IFL
Prim-25 dorsal aorta IFL
Prim-25 epithelial cell nucleus IFL
Prim-25 intersegmental vessel IFL
y1Tg standard conditions 26+ somites dorsal aorta IFL
Prim-5 dorsal aorta IFL
Prim-5 intersegmental vessel IFL
Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-25 dorsal aorta IFL
Prim-25 intersegmental vessel IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tjp1 IHC y1Tg standard conditions 26+ somites dorsal aorta bicellular tight junction
IHC 26+ somites notochord
IHC 26+ somites somite bicellular tight junction
IHC Prim-5 dorsal aorta bicellular tight junction
IHC Prim-5 intersegmental vessel bicellular tight junction
IHC Prim-5 notochord
IHC Prim-5 somite bicellular tight junction
IHC Prim-15 dorsal aorta bicellular tight junction
IHC Prim-15 intersegmental vessel bicellular tight junction
IHC Prim-15 notochord
IHC Prim-15 somite bicellular tight junction
IHC Prim-25 dorsal aorta bicellular tight junction
IHC Prim-25 intersegmental vessel bicellular tight junction
IHC Prim-25 notochord
IHC Prim-25 somite bicellular tight junction
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 316(2), Blum, Y., Belting, H.G., Ellertsdottir, E., Herwig, L., Lüders, F., and Affolter, M., Complex cell rearrangements during intersegmental vessel sprouting and vessel fusion in the zebrafish embryo, 312-322, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.