ZFIN ID: ZDB-FIG-080323-11
Li et al., 2007 - Localized rbp4 expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development. BMC Developmental Biology   7(1):117 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4 Analyses of liver and pancreas development in Rbp4 morphants. (A-D) Dorsal view of transferrin expression at 48 hpf in a control embryo (A) and in 48 hpf embryos injected with increasing dosage of Spl MO as indicated (B-D). The midline is indicated by a horizontal point/dash line. (E, F) Cross sections of the control embryo in (A) and morphant in (C) respectively. The section plane is indicated in (A, C) by the vertical dash line. Dashed circles in (E, F) represent gut and the vertical poin/dash lines indicate the midline. (G, H) Lateral view of shh expression in 24 hpf control embryo (G) and morphant (H). (I, J) Dorsal view of GFP expression in the principle islet of pancreas in 48 hpf control Tg(ins:gfp) embryo (I) and morphant (J). (K-N) Control and morphant stained using two-color WISH with fluorescein-labeled somatostatin 2(red) and Dig-labeled elaA (blue). Panels (K, M) shows dorsal views of 3 dpf control and morphant respectively. Panels (L, N) are cross section at the planes as indicated in (K, M). Abbreviations: elaA, elastaseA; sst2, somatostatin 2; shh, sonic hedgehog; tf, transferrin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ela3l WT control Protruding-mouth exocrine pancreas ISH
WT + MO2-rbp4 standard conditions Protruding-mouth exocrine pancreas ISH
GFP zf5Tg control Long-pec endocrine pancreas IFL
zf5Tg + MO2-rbp4 standard conditions Long-pec endocrine pancreas IFL
shha WT control Prim-5 floor plate ISH
Prim-5 notochord ISH
WT + MO2-rbp4 standard conditions Prim-5 floor plate ISH
Prim-5 notochord ISH
sst2 WT control Protruding-mouth endocrine pancreas ISH
WT + MO2-rbp4 standard conditions Protruding-mouth endocrine pancreas ISH
tfa WT control Long-pec liver ISH
WT + MO2-rbp4 standard conditions Long-pec liver ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-rbp4 standard conditions Long-pec gut morphology, normal
Long-pec liver development disrupted, abnormal
Long-pec liver primordium duplicated, abnormal
WT + MO2-rbp4 standard conditions Protruding-mouth pancreas present, normal
zf5Tg + MO2-rbp4 standard conditions Long-pec pancreas present, normal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.