ZFIN ID: ZDB-FIG-071025-4
Mann et al., 2007 - Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal muscle, brain and notochord. Gene expression patterns : GEP   7(8):827-836 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: 14-19 somites

Fig. 3 Hedgehog signalling is required for appropriate regulation of vgl-2b. In-situ mRNA hybridisation for vgl-2b (blue/purple) shown anterior to top in dorsal (A–I, L, and M) or lateral (J, and K) flatmount or dorsal (N) or lateral (O and P) wholemount. Arrowheads indicate lack of vgl-2b mRNA in adaxial cells. (A–C and J–M) Smoothened (siblings, A, J, L; smo, B, K, M) and cyclopamine-treated (cycl; C) embryos in which loss of hedgehog (Hh) signalling prevents adaxial slow myogenesis. Notochord expression is unaffected. (D–F) Neither prdm1 mutation (ubo, D) nor prdm1 morpholino injection (E and F) noticeably alters vgl-2b expression. (G and H) Mutations in deltaD (aei; G) or tbx24 (fss; H) affect somite borders, but vgl-2b expression persists in slow fibres (arrowheads), although poorly segmented. (I) Floatinghead (flh) mutants have an absence of notochord due to mutations in the homologue of Xenopus Xnot and lack both notochord and early adaxial expression of vgl-2b. (N–P) Fast fibres are reduced (brackets) at 24 hpf by loss of fgf8 in acerebellar (ace) mutants, but vgl-2b expression is otherwise present.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
noto fgf8ati282a/ti282a standard conditions 20-25 somites to Prim-5 fast muscle cell ISH
vgll2b WT chemical treatment: Cyclopamine 14-19 somites Not Detected adaxial cell ISH
14-19 somites notochord ISH
dldtr233/tr233 standard conditions 14-19 somites adaxial cell ISH
14-19 somites notochord ISH
noton1/n1 standard conditions 14-19 somites Not Detected adaxial cell ISH
14-19 somites Not Detected notochord ISH
prdm1atp39/tp39 standard conditions 14-19 somites adaxial cell ISH
14-19 somites notochord ISH
smob641/b641 standard conditions 14-19 somites Not Detected adaxial cell ISH
14-19 somites notochord ISH
26+ somites Not Detected adaxial cell ISH
26+ somites notochord ISH
tbx6te314a/te314a standard conditions 14-19 somites adaxial cell ISH
14-19 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dldtr233/tr233 standard conditions 14-19 somites notochord undulate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 7(8), Mann, C.J., Osborn, D.P., and Hughes, S.M., Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal muscle, brain and notochord, 827-836, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns