Nkds inhibit early canonical Wnt/β-catenin signaling. (B) nkd1 or nkd2a injected within 7 min of fertilization and assayed at sphere stage for mkp3 expression. (A) Uninjected siblings. (D) Injection of either nkd1 or nkd2a within 7 min of fertilization and assayed at 50% epiboly for gsc expression. Arrowheads identify embryos with expanded gsc expression and an asterisk identifies embryos with a decrease in gsc expression. (C) Uninjected siblings. (E) Injection of nkd2 MO at 1–2 cell stage and assayed at sphere stage for mkp3 expression. (F) nkd2 splice site MO control. This data is quantified in panel L. (H) nkd1 MO injected at the 1–2 cell stage and assayed at 30% epiboly for gsc expression. (G) Uninjected siblings. (I) nkd2 MO injected embryos at 1–2 cell stage and assayed for gsc expression at 30% epiboly. (J) A combination of nkd1 MO and nkd2 MOs were injected at the 1–2 cell stage and assayed at 30% epiboly for gsc expression. (K) A graph of the arc of gsc expression at 30% epiboly under different conditions. Error bars represent standard deviation (Students t-test, *, p < 0.05; **, p < 0.001; ***, p < 0.0001). n = number of injected embryos. (A–F) Dorsal view; (G–J) Animal view, dorsal is down.
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