ZFIN ID: ZDB-FIG-070802-12
Schlueter et al., 2007 - Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival. Developmental Biology   305(1):377-387 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 1 Igf1rb is required to establish normal primordial germ cell (PGC) numbers at the prim-5 stage. Immunohistochemical identification of PGCs (Vasa-positive cells, arrows) in prim-5 stage zebrafish embryos, after ablation of Type-1 IGF receptors (igf1ra, igf1rb): (A) control (non-targeting) morpholinos; (B) igf1ra-MO; (C) igf1rb-MO; (D) igf1ra-MO + igf1rb-MO. Magnification, 200x. Mean PGC numbers of treatment groups are summarized in Table 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddx4 WT control Prim-5 primordial germ cell IHC
WT + MO1-igf1ra + MO1-igf1rb + MO2-igf1ra + MO2-igf1rb standard conditions Prim-5 primordial germ cell IHC
WT + MO1-igf1ra + MO2-igf1ra standard conditions Prim-5 primordial germ cell IHC
WT + MO1-igf1rb + MO2-igf1rb standard conditions Prim-5 primordial germ cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-igf1ra + MO1-igf1rb + MO2-igf1ra + MO2-igf1rb standard conditions Prim-5 primordial germ cell decreased amount, abnormal
WT + MO1-igf1rb + MO2-igf1rb standard conditions Prim-5 primordial germ cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 305(1), Schlueter, P.J., Sang, X., Duan, C., and Wood, A.W., Insulin-like growth factor receptor 1b is required for zebrafish primordial germ cell migration and survival, 377-387, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.