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ZFIN ID: ZDB-FIG-070718-7
Horsfield et al., 2007 - Cohesin-dependent regulation of Runx genes. Development (Cambridge, England)   134(14):2639-2649 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Long-pec

Fig. 7 Zebrafish cohesin functions in sister chromatid cohesion and is necessary for normal expression of Runx genes in early embryogenesis. (A-C) Mitotic defects in nz171 embryos. Confocal immunofluorescence images, stained for Rad21 (green) and α-tubulin (red), with DNA stained with DAPI (blue), as indicated on top of the panels; embryo identity is on the left. By 48 h.p.f., Rad21 protein is absent from nz171 embryos, and multiple cells have arrested in mitosis (C-C''). (D,E) Knocking down the function of Rad21 (D-D'') or Smc3 (E-E'', exon 5 splice site) reproduces the mitotic phenotype observed in nz171 embryos at 48 h.p.f. Confocal immunofluorescence images, stained for α-tubulin (red), with DNA stained with DAPI (blue), as indicated in the panels (top); embryo identity is on the left. For A-E, images are of flat-mounted embryo tails; scale bar is 30 ┬Ám. (F-I) Loss of runx1 and runx3 expression in smc3 morphants. Flat-mounted 10-somite embryos, anterior to the left. smc3 morphants (exon 2 splice site) show loss of runx1 expression in the PLM (H), and runx3 expression in the RB cells (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
runx1 AB standard conditions 10-13 somites posterior lateral plate mesoderm ISH
AB + MO5-smc3 standard conditions 10-13 somites Not Detected posterior lateral plate mesoderm ISH
runx3 AB standard conditions 10-13 somites Rohon-Beard neuron ISH
AB + MO5-smc3 standard conditions 10-13 somites Not Detected Rohon-Beard neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-rad21a standard conditions Long-pec cell spindle morphology, abnormal
Long-pec mitotic cell cycle arrested, abnormal
Long-pec mitotic sister chromatid cohesion process quality, abnormal
AB + MO6-smc3 standard conditions Long-pec cell spindle morphology, abnormal
Long-pec mitotic cell cycle arrested, abnormal
Long-pec mitotic sister chromatid cohesion process quality, abnormal
rad21anz171/nz171(AB) standard conditions 14-19 somites cell spindle morphology, abnormal
14-19 somites mitotic cell cycle arrested, abnormal
14-19 somites mitotic sister chromatid cohesion process quality, abnormal
Long-pec cell spindle morphology, abnormal
Long-pec mitotic cell cycle arrested, abnormal
Long-pec mitotic sister chromatid cohesion process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development