ZFIN ID: ZDB-FIG-070421-4
Shankaran et al., 2007 - Completing the set of h/E(spl) cyclic genes in zebrafish: her12 and her15 reveal novel modes of expression and contribute to the segmentation clock. Developmental Biology   304(2):615-632 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 14-19 somites

Fig. 5 Delta–Notch control of her12 and her15 expression. her12 expression was examined in Su(H) morphants (2 experiments, n = 125, 96% affected), after DAPT treatment (2 experiments, n = 41, 98% affected), in the fused somite type mutants aei/deltaD, bea/deltaC, des/notch1a and in her7 morphants (2 experiments, n = 93, 85% affected) and compared to the respective wild type expression. Similarly, her15 expression was analysed in Su(H) morphants (3 experiments, n = 21, 100% affected), after DAPT treatment (2 experiments, n = 37, 98% affected), in the fused somite type mutants aei/deltaD, bea/deltaC, des/notch1a and in her7 morphants (2 experiments, n = 74, 69% affected) and compared to the respective wild type expression. Wild type (wt) expression of her12 and her15 (A and H, respectively). Expression of her12 and her15 in Su(H) morphants (B and I), after DAPT treatment (C and J), in aei (D and K) in bea (E and L) and in des (F and M, respectively) at the 10- to 12-somite stage. (G and N) her12 and her15 expression in her7 morphants, respectively. (O–R) (S–V) her15 expression in aei, bea, des and Su(H) morphants at the 12- to 14-somite stage (in lateral view) and at bud stage, respectively. White arrowhead points to the ventral expression domain of her15 that is only lost in the aei mutant situation.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
her12 WT standard conditions 10-13 somites central nervous system ISH
WT chemical treatment: DAPT 10-13 somites segmental plate ISH
WT + MO4-her7 standard conditions 10-13 somites segmental plate ISH
WT + MO4-rbpja,rbpjb standard conditions 10-13 somites segmental plate ISH
dlctm98/tm98 standard conditions 10-13 somites central nervous system ISH
10-13 somites segmental plate ISH
dldtg249/tg249 standard conditions 10-13 somites central nervous system ISH
10-13 somites segmental plate ISH
notch1atp37/tp37 standard conditions 10-13 somites central nervous system ISH
10-13 somites Not Detected segmental plate ISH
her15.1 WT standard conditions 10-13 somites central nervous system ISH
10-13 somites segmental plate ISH
WT chemical treatment: DAPT 10-13 somites segmental plate ISH
WT + MO4-her7 standard conditions 10-13 somites segmental plate ISH
WT + MO4-rbpja,rbpjb standard conditions Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites to 14-19 somites segmental plate ISH
dlctm98/tm98 standard conditions Bud segmental plate ISH
10-13 somites central nervous system ISH
10-13 somites segmental plate ISH
10-13 somites to 14-19 somites segmental plate ISH
dldtg249/tg249 standard conditions Bud segmental plate ISH
10-13 somites central nervous system ISH
10-13 somites segmental plate ISH
10-13 somites to 14-19 somites segmental plate ISH
notch1atp37/tp37 standard conditions Bud segmental plate ISH
10-13 somites central nervous system ISH
10-13 somites segmental plate ISH
10-13 somites to 14-19 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 304(2), Shankaran, S.S., Sieger, D., Schroter, C., Czepe, C., Pauly, M.C., Laplante, M.A., Becker, T.S., Oates, A.C., and Gajewski, M., Completing the set of h/E(spl) cyclic genes in zebrafish: her12 and her15 reveal novel modes of expression and contribute to the segmentation clock, 615-632, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.