ZFIN ID: ZDB-FIG-060627-3
Sieger et al., 2006 - her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo. Developmental Biology   293(1):242-251 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 10-13 somites

Fig. 3 Expression of myoD and somite border effects in the different morphants. (A) Wild-type expression of myoD, (B) in her1 morphants (0.6 mM; 2 experiments, n = 40, 95% show segmental myoD expression, which is slightly more diffuse in anterior somites), (C) in her13.2 morphants (0.6 mM; 2 experiments, n = 38, all embryos show segmental myoD expression) and (D) a full disruption of the myoD pattern in her1/her13.2 morphants (0.6 mM her1Mo + 0.6 mM her13.2Mo; 2 experiments, n = 57, 96.49% affected). Somite border disruption in (E) beatm98/deltaC mutants (van Eeden et al., 1996 and Jülich et al., 2005b), (F) her13.2Mo injections in beatm98/deltaC mutant background (0.6 mM; 2 experiments, n = 65, all embryos show bea phenotype), (G) Su(H) morphants (0.6 mM; 1 experiment, n = 40, 95% affected, showing the Su(H)Mo phenotype (Sieger et al., 2003)) and (H) Su(H)/her13.2 double morphants (0.6 mM Su(H)Mo + 0.6 mM her13.2Mo; 2 experiments, n = 52, 96.15% show Su(H)Mo phenotype). No enhanced effects could be observed in panels (F) and (H) when her13.2 morpholino was additionally injected compared to the single beatm98 mutant (E) or Su(H) morphant situation (G), respectively. (A–D) 8–10 somite stage embryos, flat mounted embryos, anterior to the top. (E–H) Whole-mount embryos, lateral view, anterior to the left. Stars in panels (E–H) mark the position of the somite borders.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT standard conditions 10-13 somites paraxial mesoderm ISH
10-13 somites somite ISH
WT + MO2-her1 standard conditions 10-13 somites paraxial mesoderm ISH
10-13 somites somite ISH
WT + MO2-her1 + MO3-hes6 standard conditions 10-13 somites paraxial mesoderm ISH
10-13 somites somite ISH
WT + MO3-hes6 standard conditions 10-13 somites paraxial mesoderm ISH
10-13 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 293(1), Sieger, D., Ackermann, B., Winkler, C., Tautz, D., and Gajewski, M., her1 and her13.2 are jointly required for somitic border specification along the entire axis of the fish embryo, 242-251, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.