FIGURE

Fig. 1

ID

ZDB-FIG-051105-1

Publication

Rieger et al., 2005 - Quantum dots are powerful multipurpose vital labeling agents in zebrafish embryos

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Fig. 1

Quantum dots provide bright vital labeling during zebrafish embryogenesis. A-D: Lateral views of embryo injected with QD605 (100 mM, 1.7 nl) at the two-cell stage. A-D: Bright red fluorescent labeling can be observed during the first cleavage stages (A, weaker labeling of descendants from the uninjected blastomere (white arrowhead) indicates spreading of the quantum dots during first cleavage stages), gastrulation (B), somitogenesis (C), and organogenesis (D) without affecting embryonic development. E: The tendency of QD605 to form aggregates (aqueous QD605 dispersion on a coverslip, see yellow arrowheads for aggregates) results in bright fluorescent clusters in some tissues (D, arrowheads). F,H: These aggregates can be dissolved by sonication (F, sonicated aqueous QD605 dispersion on coverslip) before embryonic injection, resulting in evenly labeled embryos (H). G: Whereas 10⁸ injected quantum dots are being tolerated by developing zebrafish embryos, higher injection amounts appear to interfere with embryogenesis. hpf, hours postfertilization.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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