Confocal images of Tg(gfap:GFP)mi2001 and the Tg(gfap:hGFAP(R239C)-GFP) transgenic zebrafish lines at 10 dpf. (a) Whole brain of a control Tg(gfap:GFP)mi2001 zebrafish larva at low magnification in dorsal view. (b,c) Enlargement of the telencephalic (b) and spinal cord (c) regions of the same larva showing the elongated pattern of GFP-positive cells (arrows). (d) Whole brain of a Tg(gfap:hGFAP(R239C)-GFP) zebrafish larva at low magnification in dorsal view. (e,f) Enlargement of the telencephalic (e) and spinal cord (f) regions of the same larva showing the presence of both GFP-positive aggregates (arrowheads) and the elongated pattern. In all panels, nuclei are shown in blue. The scale bar is 100 ?m in panels a and d, and 10 ?m in all other panels. Abbreviations: Cb, cerebellum; MO, medulla oblongata; OB, olfactory bulb; SC, spinal cord; Tel, telencephalon; TeO, tectum opticum. Plasmids used to generate transgenic lines are shown in (g).

Expression of GFP in the Tg(gfap:hGFAP(R239C)-GFP) transgenic zebrafish line at 24 hpf and 10 dpf. (a) Side view of a 24 hpf embryo. (b) anterior dorsal view of a 10 dpf larva, while (c) is a lateral view of the posterior part of the same larva. (d?s) GFP expression in larvae of Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) and Tg(gfap:GFP)mi2001 (indicated as gfap:GFP) at 10 days post-fertilization (dpf) was analyzed alongside comparisons to glial and neuronal markers in cryosections of the brain. (d) Immunostaining with anti-HuC/D (red) revealed labeling of ventrolateral neurons in the brain, distinct from GFP-positive cells (e). Merged imaging in (g) confirmed the absence of colocalization between anti-HuC/D and GFP signals. (h) Anti-GFAP staining (red) highlights intermediate filaments in the brain. (t) Cells labeled with anti-GFAP also exhibited GFP expression (green), as depicted in the overlay image (k), indicating co-localization of anti-GFAP and GFP signals. (l?o) Immunostaining with anti-HuC/D (l, red) revealed labeling of ventrolateral neurons in the brain of gfap:GFP control line, distinct from GFP-positive cells lining the ventricle and extending processes towards the pial surface (m, green). Neither colocalization was visible in merged images (o). (p-s) Cells in the brain stained with the GFAP antibody (p) colocalized with GFP expressing cells (q,s). Labeled nuclei by Hoechst are shown in panels f, j, n, r. The scale bar is 50 ?m in panels a, d-s and 100 ?m in panels c, b.

(a) Scatterplot showing the most significant GO terms emerging from the RNA-seq in the zebrafish Tg(gfap:hGFAP(R239C)-GFP) larvae compared to Tg(gfap:GFP)mi2001 controls. Analysis was performed with Revigo, which uses multidimensional scaling (MDS) to reduce the dimensionality of a matrix of the GO terms pairwise semantic similarities. The resulting projection may be highly nonlinear. Connected similar GO terms remain close together in the plot; the semantic space units have no intrinsic meaning. Bubble color indicates the p-value for the false discovery rates; circle size indicates the frequency of the GO term in the underlying GO database, where bubbles of more general terms are larger. (b) Lipid peroxidation accumulation during animal development. The graph shows the intracellular MDA level in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae from 24 hpf to 20 dpf. Data represent 3 independent experiments (n = 3), and, for each experiment, n = 25 larvae were employed for each sample group). Statistical analysis between the three sample groups was performed with one-way ANOVA followed by Tukey's multiple comparisons test, for each time point analyzed. The difference between a time points and the previous one in the R239C-GFP sample was assessed with the t-test. Statistical significance between groups is reported as follows: ????/#### < 0.0001; ? indicates comparisons between R239C-GFP and wt; # indicates comparisons between the animal's age and previous age in the same condition.

Whole-mount in situ hybridization (ISH) with miR-124 LNA (Locked Nucleic Acid) probe on 24-hpf, 72-hpf and 10 dpf. Black arrows show presence (a, b, c) or absence (d, e, f) of miR-124 expression. Scale bar is 50 ?m.

(a) Scatterplot showing the most significant GO terms emerging from proteomic analysis at 10 dpf in the zebrafish Tg(gfap:hGFAP(R239C)-GFP) larvae compared to Tg(gfap:GFP)mi2001 controls. (b) Scatterplot showing the most significant GO terms emerging from proteomic analysis of 20-dpf Tg(gfap:hGFAP(R239C)-GFP) and Tg(gfap:GFP)mi2001 larvae. Analysis was performed with Revigo, which uses Multidimensional Scaling (MDS) to reduce the dimensionality of a matrix of the GO terms pairwise semantic similarities. The resulting projection may be highly nonlinear. Connected similar GO terms remain close together in the plot; the semantic space units have no intrinsic meaning. Bubble color indicates the p-value for the false discovery rates; circle size indicates the frequency of the GO term in the underlying GO database, where bubbles of more general terms are larger.

Venn diagram, obtained with PANGEA, illustrating the biological processes emerging from proteomics analysis at 10 and 20 dpf in the mutant Tg(gfap:hGFAP(R239C)-GFP) compared to the control.

(a) ATP synthesis, oxygen consumption rate (OCR), and the ratio between ATP synthesis and oxygen consumption (P/O) in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf, induced by pyruvate plus malate (P/M) or succinate (Succ) as respiring substrates. Each panel is representative of n = 3 independent experiments and, for each experiment, n = 25 larvae were employed for each sample group. (b) 3-hydroxyacyl-CoA dehydrogenase and ?-ketoacyl-ACP reductase activity in wild-type (wt), Tg(gfap:GFP)mi2001(indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf. Each panel is representative of n = 6 independent experiments and, for each experiment, n = 25 larvae were employed for each sample group. (c) Superoxide levels (n = 3 independent experiments), and NADPH oxidase activity (n = 6 independent experiments) in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf. For each experiment, n = 25 larvae were employed for each sample group. (d) Catalase activity, glutathione reductase activity, and glutathione peroxidase activity in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf. Panels are representative of n = 6 independent experiments, and, for each experiment, n = 25 larvae were employed for each sample group. Statistical analysis between the three sample groups was performed with one-way ANOVA followed by Tukey's multiple comparisons test, for each larvae stage. Statistical analysis on Tg(gfap:hGFAP(R239C)-GFP) between 10 or 20 dpf stages was performed by t-test. Statistical significance between groups is reported as follows: ns = not significant; ?/# = 0,01; ??/## = 0,001; ???/### = 0,0001; ????/#### <0,0001. ? = p value vs wt; # = p value R239C-GFP 10 dpf vs 20 dpf.