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Fig. 7 (a) ATP synthesis, oxygen consumption rate (OCR), and the ratio between ATP synthesis and oxygen consumption (P/O) in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf, induced by pyruvate plus malate (P/M) or succinate (Succ) as respiring substrates. Each panel is representative of n = 3 independent experiments and, for each experiment, n = 25 larvae were employed for each sample group. (b) 3-hydroxyacyl-CoA dehydrogenase and ?-ketoacyl-ACP reductase activity in wild-type (wt), Tg(gfap:GFP)mi2001(indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf. Each panel is representative of n = 6 independent experiments and, for each experiment, n = 25 larvae were employed for each sample group. (c) Superoxide levels (n = 3 independent experiments), and NADPH oxidase activity (n = 6 independent experiments) in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf. For each experiment, n = 25 larvae were employed for each sample group. (d) Catalase activity, glutathione reductase activity, and glutathione peroxidase activity in wild-type (wt), Tg(gfap:GFP)mi2001 (indicated as gfap:GFP), and Tg(gfap:hGFAP(R239C)-GFP) (indicated as R239C-GFP) larvae at 10 and 20 dpf. Panels are representative of n = 6 independent experiments, and, for each experiment, n = 25 larvae were employed for each sample group. Statistical analysis between the three sample groups was performed with one-way ANOVA followed by Tukey's multiple comparisons test, for each larvae stage. Statistical analysis on Tg(gfap:hGFAP(R239C)-GFP) between 10 or 20 dpf stages was performed by t-test. Statistical significance between groups is reported as follows: ns = not significant; ?/# = 0,01; ??/## = 0,001; ???/### = 0,0001; ????/#### <0,0001. ? = p value vs wt; # = p value R239C-GFP 10 dpf vs 20 dpf.