Fig. 1. Absence of scavenger receptors stab1 and/or stab2 reduces oxLDL uptake by endothelial cells.

(A) Schematics of experimental set-up; injection of fluorescent labeled lipoproteins in 3 dpf ABTL or stabilin knockout zebrafish larvae. (B) Photomicrographs showing an overview of 3 dpf ABTL, stab1^−/−, stab2^−/−, and stab1^−/−stab2^−/− zebrafish larvae after injection of oxLDL (0.5 mg/mL; green). (C) Uptake of oxLDL (0.5 mg/mL; green) in the caudal vein of 3dpf ABTL, stab1^−/−, stab2^−/− and stab1^−/−stab2^−/− zebrafish larvae. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2. Absence of scavenger receptors stab1 and stab2 reduces ApoB containing lipoprotein clearance by endothelial cells.

(A–C) Uptake of lipoproteins by endothelial cells and macrophages in 3dpf ABTL and stab1^−/−stab2^−/− mpeg1:rfp transgenic zebrafish at 3 h post injection (hpi) ((ox)LDL) or 24 hpi (HDL) and the quantification of co-localization events between lipoproteins (green) and macrophages (purple) after 1 hpi (n = 9), 3 hpi (n = 6) or 24 hpi (n = 10). (D) Overview of possible mechanisms regarding cellular cholesterol uptake in ABTL and stab1^−/−stab2^−/−. Means ± SEM; p < 0.01**; p < 0.001***. Magnification: 10× and 40×. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 3. Stabilin 1 and 2 deficiency does not affect genes involved in cholesterol synthesis and uptake in 3 days post fertilization old zebrafish larvae.

The effect of stab1 and 2 deficiency on the relative expression of genes (A) involved in scavenging (B) cholesterol metabolism (C) and cholesterol efflux. Means ± SEM (n = 7, pool of 5); p < 0.05*.

Fig. 4. Stabilins are an important player in zebrafish total-body cholesterol homeostasis after challenged with a 10 day high cholesterol diet (HCD).

(A) Relative expression of stab1 and 2 in ABTL zebrafish (15 dpf old) after 10 day normal (CTRL) or either 10 day high cholesterol diet (HCD). (B) Whole body total cholesterol levels in 15dpf ABTL and stab1^−/−stab2^−/− zebrafish fed with a HCD for 10 days. (C) The effect of stab1 and 2 deficiency after 10 days of HCD on relative expression of genes involved in cholesterol acquisition (D) scavenging and (E) cholesterol efflux. Means ± SEM (n = 7; pool of 5 fish); p < 0.01**; p < 0.001***.