Distribution of the CTCF protein through development. Whole-mount immunoflourescence staining of zebrafish embryos, from the 1-cell to the prim-16 stages (staging according to Kimmel et al., 1995), encompassing the first 30 h of development.

Mutations induced in the Ctcf gene by the CRISPR-Cas9 system. Embryos at the 1-cell stage were injected with the CRISPR-Cas9 system and compared to uninjected embryos or to Cas9 (without the sgRNA) injected embryos. A. Scheme of a portion of the ctcf gene. The sgRNA target site and the site of the different deletions (Δ) and the insertion (+) found in the sequenced clones from the CRISPR-Cas9 injected embryos are indicated. B. Percentage of the CRISPR-Cas9 injected embryos that resulted in lethality, the phenotype shown in C or wild type morphology. C. Representative images of the phenotype found in the CRISPR-Cas9 injected embryos compared to Cas9 injected controls in the bud stage.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Phenotype of the CTCF morphants. 1-cell to 2-cell embryos were injected with mo-aug or mo-sp. A range of phenotypes was found at 24 hpf, from wild type, mild and severe phenotypes. Different structures were affected: a. Forebrain, b. Midbrain, c. Hindbrain, d. Eye, e. Notochord f. Somites. The percentages and the median of embryos with a mild or severe phenotype are shown in the graph. The statistical difference is noted with an asterisk (n = 4 assays, p = 0.006 for mo-aug; n = 9 assays, p = 0.2609 for mo-sp; one-tailed unpaired t-tests).

Rescue of the morphant phenotype by CTCF mRNA injection. Embryos were injected only with mo-aug (mo) or mo and either 50 pg or 100 pg of the human in vitro transcribed CTCF mRNA, and photographed at 24 hpf. The regions affected with the mo, and partially rescued with the CTCF mRNA are indicated: a. Forebrain, b. Midbrain, c. Hindbrain, d. Eye, e. Notochord, f. Somites. The percentage of lethal or wild type embryos, or embryos with the phenotype described in Fig. 4, was graphed.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.