FIGURE

Fig. 2

ID

ZDB-FIG-190328-33

Publication

Carmona-Aldana et al., 2018 - CTCF knockout reveals an essential role for this protein during the zebrafish development

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Fig. 2

Mutations induced in the Ctcf gene by the CRISPR-Cas9 system. Embryos at the 1-cell stage were injected with the CRISPR-Cas9 system and compared to uninjected embryos or to Cas9 (without the sgRNA) injected embryos. A. Scheme of a portion of the ctcf gene. The sgRNA target site and the site of the different deletions (Δ) and the insertion (+) found in the sequenced clones from the CRISPR-Cas9 injected embryos are indicated. B. Percentage of the CRISPR-Cas9 injected embryos that resulted in lethality, the phenotype shown in C or wild type morphology. C. Representative images of the phenotype found in the CRISPR-Cas9 injected embryos compared to Cas9 injected controls in the bud stage.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-ctcf
Knockdown Reagent:	CRISPR1-ctcf
Observed In:	head post-vent region whole organism
Stage Range:	Bud to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 154, Carmona-Aldana, F., Zampedri, C., Suaste-Olmos, F., Murillo-de-Ozores, A., Guerrero, G., Arzate-Mejía, R., Maldonado, E., Navarro, R., Chimal-Monroy, J., Recillas-Targa, F., CTCF knockout reveals an essential role for this protein during the zebrafish development, 51-59, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.