Fig. 2

Fig. 2 Mutations induced in the Ctcf gene by the CRISPR-Cas9 system. Embryos at the 1-cell stage were injected with the CRISPR-Cas9 system and compared to uninjected embryos or to Cas9 (without the sgRNA) injected embryos. A. Scheme of a portion of the ctcf gene. The sgRNA target site and the site of the different deletions (Δ) and the insertion (+) found in the sequenced clones from the CRISPR-Cas9 injected embryos are indicated. B. Percentage of the CRISPR-Cas9 injected embryos that resulted in lethality, the phenotype shown in C or wild type morphology. C. Representative images of the phenotype found in the CRISPR-Cas9 injected embryos compared to Cas9 injected controls in the bud stage.