FIGURE SUMMARY
Title

Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy

Authors
Bancelin, S., Couture, C.A., Pinsard, M., Rivard, M., Drapeau, P., Légaré, F.
Source
Full text @ Sci. Rep.
Fig. 1

Time-lapse SHG imaging of mitotic spindles during mitotic division of a zebrafish embryo. Prometaphase (P; 20?160?s), metaphase (M; 180?300?s), anaphase (320?400?s) and telophase (420?480?s). Average power at focus: 150?mW, photomultiplier tube set at 1100?V. Scale bar: 10?µm.
Fig. 2

I-SHG of mitotic spindles in a zebrafish embryo. (a?c) Images acquired with different reference phases (0°, 90° and 180° respectively). Constructive and destructive interference occurs in opposite poles at 0° and 180° while no variations are observable at 90°. (d) Subtraction of the images (a,c) showing the interferometric contrast in the two poles. (e) 2-colors merge of the images acquired at 0° (in red) and 180° (in green). Average power at focus: 100?mW, photomultiplier tube set at 1050?V. Field of view: 50?×?50?µm2.
Fig. 3

Image and histogram of the phase of the SHG signal generated in the spindles during mitosis. Field of view: 29?×?29?µm2.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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