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Fig. 2
I-SHG of mitotic spindles in a zebrafish embryo. (a–c) Images acquired with different reference phases (0°, 90° and 180° respectively). Constructive and destructive interference occurs in opposite poles at 0° and 180° while no variations are observable at 90°. (d) Subtraction of the images (a,c) showing the interferometric contrast in the two poles. (e) 2-colors merge of the images acquired at 0° (in red) and 180° (in green). Average power at focus: 100 mW, photomultiplier tube set at 1050 V. Field of view: 50 × 50 µm2.
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