FIGURE SUMMARY
Title

Identification of Spt5 target genes in zebrafish development reveals its dual activity in vivo

Authors
Krishnan, K., Salomonis, N., and Guo, S.
Source
Full text @ PLoS One
Fig. 1

Spt5 protein is significantly decreased in fogsk8 embryos.

(A–B) Whole mount immunofluorescence detection of Spt5 and RNAPII in zebrafish embryos, showing the nuclear localization pattern of Spt5 protein. (C–D) Whole mount immunofluorescence detection of Spt5 in WT and fogsk8 embryos at 24 hpf, showing overall decrease of Spt5 protein in fogsk8 embryos. Confocal images of lateral views are shown, with the anterior to the left and dorsal up. (E) Western blot of wild-type (WT) and fogsk8 embryonic extracts at 19 hpf and 24 hpf shows substantial decrease in maternal Spt5 protein in the mutant. (F–G) Light microscopic view of WT and sk8 embryos at 24 hpf, showing the gross morphological phenotype of the mutant embryos.

EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5
Fig. 8

Global increase of gadd45 mRNA and increased Gadd45b protein in fogsk8 mutant embryos.

(A–H) Whole-mount in situ hybridization using the gadd45b antisense probe in WT and fogsk8 embryos at 16 hpf and 24 hpf. gadd45b expression is not increased at 16 hpf but shows widespread increase at 24 hpf in fogsk8 embryos. (I) Western blot analysis of WT and fogsk8 embryo extracts with commercially available anti-Gadd45b antibody and anti-Actin antibody shows significant increase of Gadd45b protein in fogsk8 embryos at 24 hpf.
Fig. S1

Whole mount in situ hybridization validation of candidate genes in WT (A, C, E, G) and fogsk8 (B, D, F, H) embryos at 24hpf. gch (A, B) and pvalb (C, D) are not expressed (357 fold and 17 fold down-regulation in the array) in fog sk8 embryos. hoxB6 (E, F) expression is up-regulated 2.2 fold while dlx2 (G, H) expression is not changed in fogsk8 embryos.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
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