Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
5	46.8 cM	z20951	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
5	147.15 cR	Z20951	Loeb/NIH/5000/4000 (LN54)	Dawid, Igor B.	Data
5	1994.0 cR	z20951	Goodfellow T51 (T51)	Geisler, Robert	Data
5	49.3 cM	z20951	Heat Shock (HS)	Woods, Ian G.	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
z22523	SSLP	5		Bland, 2001	Bland (2001) mapped bmp10 onto the LN54 panel between the SSLP markers z22523  ... Bland (2001) mapped bmp10 onto the LN54 panel between the SSLP markers z22523 and z2095l on LG5.
bmp10	GENE	5		Bland, 2001	Bland (2001) mapped bmp10 onto the LN54 panel between the SSLP markers z22523  ... Bland (2001) mapped bmp10 onto the LN54 panel between the SSLP markers z22523 and z2095l on LG5.
cemip2	GENE	5		Smith et al., 2011	Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles  ... Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles hu4800 and hu5935 to a genetic interval of 0.3 Mb on LG5 between markers z42819b and z20951 on the MGH mapping panel, with subsequent sequencing confirming mutant localization to tmem2.
hu4800	Feature	5		Smith et al., 2011	Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles  ... Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles hu4800 and hu5935 to a genetic interval of 0.3 Mb on LG5 between markers z42819b and z20951 on the MGH mapping panel, with subsequent sequencing confirming mutant localization to tmem2.
hu5935	Feature	5		Smith et al., 2011	Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles  ... Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles hu4800 and hu5935 to a genetic interval of 0.3 Mb on LG5 between markers z42819b and z20951 on the MGH mapping panel, with subsequent sequencing confirming mutant localization to tmem2.
z42819b	SSLP	5		Smith et al., 2011	Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles  ... Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles hu4800 and hu5935 to a genetic interval of 0.3 Mb on LG5 between markers z42819b and z20951 on the MGH mapping panel, with subsequent sequencing confirming mutant localization to tmem2.

OTHER MAPPING INFORMATION

Chr 5 Bland, 2001 Bland (2001) mapped bmp10 onto the LN54 panel between the SSLP markers z22523 and z2095l on LG5. Bland (2001) mapped bmp10 onto the LN54 panel between the SSLP markers z22523 and z2095l on LG5.   Chr 5 Smith et al., 2011 Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles hu4800 and hu5935 to a  ... Smith, et al. (2011. Development 138(19):4193-4198.) reports mapping alleles hu4800 and hu5935 to a genetic interval of 0.3 Mb on LG5 between markers z42819b and z20951 on the MGH mapping panel, with subsequent sequencing confirming mutant localization to tmem2.

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
AB	140		60.0
Forward Primer	TCCCACACAGAATGTCTCCA
Reverse Primer	TCAACGCCATCAGAGATGTC
IND	0,142		60.0
Forward Primer	TCCCACACAGAATGTCTCCA
Reverse Primer	TCAACGCCATCAGAGATGTC
TU	0,138		60.0
Forward Primer	TCCCACACAGAATGTCTCCA
Reverse Primer	TCAACGCCATCAGAGATGTC
EKW	0,138		60.0
Forward Primer	TCCCACACAGAATGTCTCCA
Reverse Primer	TCAACGCCATCAGAGATGTC