Donizetti et al., 2015 - Expression pattern of zebrafish rxfp2 homologue genes during embryonic development. Journal of experimental zoology. Part B, Molecular and developmental evolution   324(7):605-13 Full text @ J. Exp. Zool. B Mol. Dev. Evol.

Fig. 1

Embryonic gene expression pattern for the three rxfp2 genes by RT-PCR analysis. The different embryonic stages are indicated on top as hours post fertilization. Amplification of rplp0 cDNA was used as a control of RT-PCR sensitivity in the assay. B and O indicate cDNA obtained by retrotranscription of RNA extracted from adult zebrafish brain and ovary respectively. C indicates the negative PCR control reaction lacking cDNA template.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Adult

Fig. 2

Spatial localization of rxfp2l transcript during zebrafish late embryogenesis. A: Detail of dorsal region of embryonic brain at 48 hpf. B: Detail of dorsal region of larval brain at 72 hpf. Black arrow indicates the specific hybridization signal. Scale bar: 50 µm. TeO, optic tectum.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Spatial localization of rxfp2l transcript in the anterior region of zebrafish larval brain. A–I: Transverse sections of hybridized zebrafish larvae (72 hpf) as indicated in the schematic drawing of the zebrafish larval brain. Scale bar: 25 µm. ac, anterior commissure; CeP, cerebellar plate; DT, dorsal thalamus; E, epiphysis; EmT, eminentia thalami; H, hypothalamus; Ha, habenula; MO, medulla oblongata; N, region of the nucleus of medial longitudinal fascicle; oc, optic chiasma; OE, olfactory epithelium; P, pallium; Po, preoptic region; poc, postoptic region; Pr; pretectum; S, subpallium; T, midbrain tegmentum; TVe, telencephalic ventricle; TeO, optic tectum; VT, ventral thalamus.

Fig. 4

Spatial localization of rxfp2l transcript in the middle region of zebrafish larval brain. A–H: Transverse sections of hybridized zebrafish larvae (72 hpf) as indicated in the schematic drawing of the zebrafish larval brain. Scale bar: 50 µm. ac, anterior commissure; CeP, cerebellar plate; DT, dorsal thalamus; E, epiphysis; EmT, eminentia thalami; H, hypothalamus; Hi, intermediate hypothalamus; Hc, caudal hypothalamus; MO, medulla oblongata; N, region of the nucleus of medial longitudinal fascicle; oc, optic chiasma; P, pallium; Po, preoptic region; poc, postoptic region; PT, posterior tuberculum; S, subpallium; T, midbrain tegmentum; TeO, optic tectum; TG, trigeminal ganglion; TS, torus semicircularis; VT, ventral thalamus.

Fig. 5

Spatial localization of rxfp2l transcript in the caudal region of zebrafish larval brain. A–F: Transverse sections of hybridized zebrafish larvae (72 hpf) as indicated in the schematic drawing of the zebrafish larval brain. Black arrowhead indicates restricted rxfp2l-expressing cell cluster in the medulla oblongata. Scale bar: 50 µm. ac, anterior commissure; CC, cerebellar crest; CeP, cerebellar plate; Ch, chorda dorsalis; DT, dorsal thalamus; E, epiphysis; EmT, eminentia thalami; H, hypothalamus; MO, medulla oblongata; N, region of the nucleus of medial longitudinal fascicle; oc, optic chiasma; OV, otic vesicle; P, pallium; Po, preoptic region; poc, postoptic region; S, subpallium; T, midbrain tegmentum; TeO, optic tectum; VT, ventral thalamus.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Protruding-mouth

Fig. 6

Spatial localization of rxfp2l transcript outside the zebrafish brain. A: Detail of transverse section showing the eye of zebrafish larvae (72 hpf). B: Magnification of inner cell layer as indicated in A. C–F: Detail of transverse sections showing the pharyngeal arches region of zebrafish larvae (72 hpf). Black arrow indicates rxfp2l-expressing cells in the epithelium. Scale bar: 50 µm. a, amacrine cells; b, bipolar cells; Ep, ethmoid plate; gcl, ganglion cell layer; h, horizontal cells; icl, inner cell layer; le, lens; tb, trabeculae.

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Zool. B Mol. Dev. Evol.