PUBLICATION
Spatiotemporal Coordination of FGF and Shh Signaling Underlies the Specification of Myoblasts in the Zebrafish Embryo
- Authors
- Yin, J., Lee, R., Ono, Y., Ingham, P.W., Saunders, T.E.
- ID
- ZDB-PUB-180927-10
- Date
- 2018
- Source
- Developmental Cell 46: 735-750.e4 (Journal)
- Registered Authors
- Ingham, Philip, Lee, Raymond, Ono, Yosuke, Saunders, Timothy Edward
- Keywords
- FGF signaling, Sonic hedgehog, adaxial cells, fast muscle fibers, in vivo imaging, myogenesis, somite polarity, somite rotation, temporal regulation, zebrafish
- MeSH Terms
-
- Myoblasts/cytology*
- Myoblasts/metabolism
- Fibroblast Growth Factors/metabolism*
- Cell Differentiation
- Zebrafish Proteins/metabolism*
- Animals
- Cells, Cultured
- Cell Lineage*
- Muscle, Skeletal/embryology*
- Zebrafish/embryology*
- Zebrafish/physiology
- Signal Transduction
- Morphogenesis
- Hedgehog Proteins/metabolism*
- PubMed
- 30253169 Full text @ Dev. Cell
Citation
Yin, J., Lee, R., Ono, Y., Ingham, P.W., Saunders, T.E. (2018) Spatiotemporal Coordination of FGF and Shh Signaling Underlies the Specification of Myoblasts in the Zebrafish Embryo. Developmental Cell. 46:735-750.e4.
Abstract
Somitic cells give rise to a variety of cell types in response to Hh, BMP, and FGF signaling. Cell position within the developing zebrafish somite is highly dynamic: how, when, and where these signals specify cell fate is largely unknown. Combining four-dimensional imaging with pathway perturbations, we characterize the spatiotemporal specification and localization of somitic cells. Muscle formation is guided by highly orchestrated waves of cell specification. We find that FGF directly and indirectly controls the differentiation of fast and slow-twitch muscle lineages, respectively. FGF signaling imposes tight temporal control on Shh induction of slow muscles by regulating the time at which fast-twitch progenitors displace slow-twitch progenitors from contacting the Shh-secreting notochord. Further, we find a reciprocal regulation of fast and slow muscle differentiation, morphogenesis, and migration. In conclusion, robust cell fate determination in the developing somite requires precise spatiotemporal coordination between distinct cell lineages and signaling pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping