PUBLICATION

Zebrafish biosensor for toxicant induced muscle hyperactivity

Authors

Shahid, M., Takamiya, M., Stegmaier, J., Middel, V., Gradl, M., Klüver, N., Mikut, R., Dickmeis, T., Scholz, S., Rastegar, S., Yang, L., Strähle, U.

ID

ZDB-PUB-160401-10

Date

2016

Source

Scientific Reports 6: 23768 (Journal)

Registered Authors

Dickmeis, Thomas, Gradl, Marion, Klüver, Nils, Middel, Volker, Mikut, Ralf, Rastegar, Sepand, Shahid, Maryam, Strähle, Uwe, Takamiya, Masanari, Yang, Lixin

Keywords

Assay systems, Transgenic organisms, Wide-field fluorescence microscopy

MeSH Terms

Biosensing Techniques/methods*
Animals, Genetically Modified
Propoxur/analysis
Propoxur/toxicity
Azinphosmethyl/analysis
Azinphosmethyl/toxicity
Galantamine/analysis
Galantamine/toxicity
Promoter Regions, Genetic
Motor Activity/drug effects*
Founder Effect
Pesticides/analysis
Pesticides/toxicity
Muscles/drug effects*
Muscles/metabolism
Animals
Mutant Chimeric Proteins/agonists
Mutant Chimeric Proteins/antagonists & inhibitors
Mutant Chimeric Proteins/genetics*
Mutant Chimeric Proteins/metabolism
Gene Expression Regulation
Green Fluorescent Proteins/agonists
Green Fluorescent Proteins/antagonists & inhibitors
Green Fluorescent Proteins/genetics*
Green Fluorescent Proteins/metabolism
Zebrafish
Dose-Response Relationship, Drug
Intracellular Signaling Peptides and Proteins/agonists
Intracellular Signaling Peptides and Proteins/antagonists & inhibitors
Intracellular Signaling Peptides and Proteins/genetics*
Intracellular Signaling Peptides and Proteins/metabolism
Notochord/drug effects
Notochord/metabolism

PubMed

27029555 Full text @ Sci. Rep.

CTD

27029555

Abstract

Robust and sensitive detection systems are a crucial asset for risk management of chemicals, which are produced in increasing number and diversity. To establish an in vivo biosensor system with quantitative readout for potential toxicant effects on motor function, we generated a transgenic zebrafish line TgBAC(hspb11:GFP) which expresses a GFP reporter under the control of regulatory elements of the small heat shock protein hspb11. Spatiotemporal hspb11 transgene expression in the musculature and the notochord matched closely that of endogenous hspb11 expression. Exposure to substances that interfere with motor function induced a dose-dependent increase of GFP intensity beginning at sub-micromolar concentrations, while washout of the chemicals reduced the level of hspb11 transgene expression. Simultaneously, these toxicants induced muscle hyperactivity with increased calcium spike height and frequency. The hspb11 transgene up-regulation induced by either chemicals or heat shock was eliminated after co-application of the anaesthetic MS-222. TgBAC(hspb11:GFP) zebrafish embryos provide a quantitative measure of muscle hyperactivity and represent a robust whole organism system for detecting chemicals that affect motor function.

Genes / Markers

Figures