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Che et al., 2025 - The Mutation of myomiR miR499 Impacts the Intermuscular Bones in Zebrafish

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Figure 2

Transcriptomic analysis of muscle tissue in WT and miR499^−/− zebrafish at 3 months old. (A) Volcano plot showing differentially expressed genes between WT and miR499^−/− zebrafish (red dots: up—regulated genes; green dots: down—regulated genes; blue dots: genes with no significant change); (B) Bubble plot of pathway enrichment analysis for differentially expressed genes (Top40). The size of bubbles represents gene count, and color represents p–value. Red indicates high expression. Green indicates low expression; (C) Hierarchical clustering of the muscle-related DEGs in miR499^−/− mutants compared with WT; (D) qPCR validation of expression levels of selected myosin—related and red muscle—specific genes. ** p < 0.01, * p < 0.05.

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