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Della Vecchia et al., 2025 - Dapagliflozin ameliorates Lafora disease phenotype in a zebrafish model

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Fig. 4

(A) qRT-PCR analysis of the autophagy-lysosomal pathway. Three independent experiments were performed per experimental group (WT controls, and epm2a^−/− and DAPA-treated epm2a^−/− larvae). Statistical analysis was performed using the ANOVA test. The values are expressed as mean ± standard error of the mean (SEM). (B) Three independent larval homogenates, from controls (n = 50), epm2a^−/− larvae (n = 50) and DAPA-treated epm2a^−/− larvae (n = 50), were tested by Western blotting for the expression of LC3 protein. The protein levels were normalized to GAPDH. Statistical analysis was performed using the ANOVA test. The values are expressed as mean ± SEM. (**** p ≤ 0.0001; ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05).

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