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Fig. 9 Expression of forebrain-forming genes in foxg1a mutants at 24 hpf. (a–d″) Expression of anterior forebrain markers (six3b, fgf8a, vax2) was examined by WISH in foxg1a mutants at 24 hpf, followed by genotyping. Wild-type embryos, heterozygotes, and homozygotes are shown from left to right for each gene. Asterisks indicate abnormal expression, with more asterisks indicating more severe anomalies. (a–c, a′–c′, a″–c″) Lateral views of the head. Left is anterior, top is dorsal. (d, d″, d‴) Front view of the head. Top is dorsal. In the lower right are shown the numbers of embryos with the indicated expression and the numbers of assessed embryos. ht, hypothalamus; et, epithalamus; mhb, midbrain–hindbrain boundary; poa, preoptic area; re, retina; te, telencephalon. Scale bar, 100 μm.