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Fig. 5 The depletion of the gut microbiota impacted the VD-regulated folate metabolism. Wild-type zebrafish were treated with antibiotics and fed diets containing 0 or 800 IU/kg VD3 for 3 wk. A: the folate content of liver was measured with microbiological assay. The gene expression of slc19a1 (A), slc46a1 (B), folr1 (C), dhfr (D), and mthfr (E) in the intestine was analyzed. Data are presented as means ± SE (n ≥ 5 fish). *P < 0.05; **P < 0.01; ns, no statistical significance. VD, vitamin D.
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Full text @ Am. J. Physiol. Endocrinol. Metab.