ZFIN Image: Song et al., 2024, Figure 9

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Figure 9

RT-qPCR analysis of gene expression in cdc27^−/− mutant and control sibling zebrafish embryos. This figure illustrates the relative mRNA levels of several key genes, including cdc27, sox9a, col2a1a, cdkn1a, tp53, gadd45aa, ccng1, and serpine1, as determined by RT-qPCR analysis. In the cdc27^−/− embryos, there is a notable decrease in the mRNA levels of cdc27, sox9a, and col2a1a, suggesting altered gene expression related to craniofacial development and cartilage formation. Conversely, the mRNA levels of cdkn1a and tp53 are elevated in the mutants, indicating potential compensatory mechanisms or stress responses. Notably, the mRNA levels of gadd45aa, ccng1, and serpine1 show no significant differences between the control siblings and cdc27^−/− embryos, implying a selective impact of the cdc27 mutation on specific gene expressions. Note: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.

Acknowledgments

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