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Ott et al., 2023 - A novel role for the chloride intracellular channel protein Clic5 in ciliary function

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Figure 4

Analyses of Clic5 in ciliogenesis, and in ciliary and glomerular function in zebrafish. (A) Anti-acetylated Tubulin immunostaining reveals reduced cilia formation in the pronephric tubules of embryos injected with SB-MO clic5 (4 ng) or TB-MO clic5b (4 ng) in comparison to Co-MO (4 ng) injected embryos at 1dpf. Representative confocal images depict the middle part of the pronephric tubule for each condition with anterior to the left. Scale bar: 10 µm. (B) Quantitative RT-PCR analyses reveals unaltered expression of Hedgehog signalling components gli1 and ptc1 while Wnt signalling components axin2, wnt8a and lef1 were upregulated upon SB-MO clic5 (4 ng) mediated knockdown compared to the control at 1dpf. (C,D) Injection of TB-MO clic5a (6 ng) leads to detectable FITC-dextran 500 kDa in the pronephric tubules at 4dpf of zebrafish development in comparison to Co-MO (6 ng) injected embryos shown by respective confocal images of Tg(cdh17:mcherry) embryos. Scale bar: 10 µm. (E) Quantification reveals statistic significant FITC-dextran 500 kDa positive embryos for the knockdown of Clic5 and Clic5a compared to the knockdown of Clic5b or the control.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.