ZFIN Image: Sugitani et al., 2023, Figure 2

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Figure 2

Upregulation of the Yamanaka factors (OSK) in zebrafish retina after ONI. (a) mRNA expression levels of OSK after ONI were determined by quantitative real-time PCR (left, klf4; center, oct4; right, sox2). (b) In situ hybridization of OSK in zebrafish retina after ONI. Klf4 mRNA expression started to increase at 1 h and localized to the GCLs at 3 h after ONI. Oct4 mRNA signal was observed in the GCL and strongly in the INL and ONL at 3 h, but this strong signal was seen in all nuclear layers at 6 h after ONI. Sox2 mRNA expression was observed in all nuclear layers 3 h after ONI and more prominent at 6 h. No positive signals could be seen with the sense probe (Sense). Five to six experiments were repeated with different retinas under each experimental condition and produced the same results. Data are expressed as the mean � SEM and analyzed by one-way ANOVA, followed by Scheffe?s multiple comparisons. Statistical significance was set at * p < 0.05. Scale bar = 50 ?m.

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Acknowledgments

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