Fig. 14

Effects of erianin on the RhoA/ROCK1 pathway in vivo. (A) The mRNA levels of rhoa, rock1I, and mypt1 in adult and embryonic zebrafish retinas exposed to normoxia, hypoxia, or erianin at various concentrations. B (B–I) Protein expression of GTP-RhoA, ROCK1, and p-MYPT1 in adult and embryonic zebrafish retinas under normoxia, hypoxia, or erianin at various concentrations. (B–II) Quantitative analysis of GTP-RhoA, ROCK1, and p-MYPT1 in adult and embryonic zebrafish retinas. The results are presented as percentages of the normoxia group. * Significant difference between samples in the adult zebrafish (paired t-test, P values < 0.05) and in the zebrafish embryos (independent sample t-test, P values < 0.05); ^# Comparison with adult zebrafish induced by low-oxygen without erianin treatment (paired t-test, P values < 0.05) or with zebrafish embryos induced by CoCl₂ without erianin treatment (independent sample t-test, P values < 0.05); † Comparison with normoxic zebrafish embryos without erianin treatment (independent sample t-test, P values < 0.05).