IMAGE

Figure 7

ID

ZDB-IMAGE-210902-197

Genes

Publication

Song et al., 2021 - Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models

All Figures

Figures for Song et al., 2021

Image

Figure Caption

Figure 7

Generation of the slc16a2 mutant zebrafish. (A) Schematic illustration representing the zebrafish Slc16a2 protein (upper panel) and slc16a2 locus with the CRISPR target site on exon 1 (middle panel). Sequencing of the slc16a2 target CRISPR site in wild type, slc16a2 (-28) mutant, and slc16a2 (-10) mutant zebrafish lines are shown in the lower panel. (B) The predicted truncated Slc16a2 proteins resulting from premature translational termination are shown. (C) Quantitative RT-PCR analyses of tshba, tg, tpo, and slc5a5 expression in slc16a2 mutants and their wild-type siblings at 5 dpf. (D) Quantitative RT-PCR analyses of slc16a2, slc16a10, and slco1c1 expressions in slc16a2 mutants and their wild-type siblings at 5 dpf. (E) Whole-body T4 contents of slc16a2 mutants and their wild-type siblings at 20, 25, and 60 dpf. (F) Whole-body T3 contents of slc16a2 mutants and their wild-type siblings at 20, 25, and 60 dpf. ** p < 0.01, ns: No significance.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells