Fig. 6

Fig. 6

Targeted disruption of ar in zebrafish causes premature ovarian failure during growth. (A-C) Gross appearance and ovaries from wildtype (ar ^+/+), heterozygous (ar ^+/-) and homozygous (ar ^-/-) (ar ^{ihb1225/ihb1225}) female zebrafish. At 4.5 mpf, the gross appearance of ar ^-/- (ar ^{ihb1225/ihb1225}) female zebrafish was indistinguishable from wildtype siblings (ar ^+/+) or their heterozygous sibling (ar ^+/-), but the ovaries of ar ^-/- female were smaller with fewer eggs and became transparent compared with wildtype sibling (ar ^+/+) or heterozygous siblings (ar ^+/-). (D) Gonadosomatic index (GSI) in ar ^+/+ and ar ^-/- female zebrafish. (E) Fertilization rate of eggs from wildtype (ar ^+/+) and homozygous (ar ^-/-) female zebrafish at 4mpf. (F, G) Histology of ovaries from wildtype (ar ^+/+) and homozygous (ar ^-/-) (ar ^{ihb1225/ihb1225}) zebrafish at 1.5 mpf. No obvious difference between ar ^-/- and ar ^+/+ ovaries. OC, Oocyte. (H, I) Histology of ovary from the wildtype (ar ^+/+) and homozygous (ar ^-/-) (ar ^{ihb1225/ihb1225}) zebrafish at 70 dpf. Ovaries of wildtype (ar ^+/+), most oocytes developed to stage III and IV; but more ovaries of homozygous (ar ^-/-) (M1) developed to stage II or I, some developed to stage III and IV. (J, K) Histology of ovaries from wildtype (ar ^+/+) and homozygous (ar ^-/-) zebrafish at 4 mpf. In ovaries of wildtype (ar ^+/+), most oocytes developed to stage IV, but for ovaries of homozygous (ar ^-/-), most oocytes developed to stage II and partial degeneration occurred. (L, M) Histology of ovaries from wildtype (ar^+/+) and homozygous (ar ^-/-) zebrafish at 4 mpf. In ovaries of homozygous (ar ^-/-), follicles atresia and degeneration occurred. (N, O) Histology of ovaries from wildtype (ar ^+/+) and homozygous (ar ^-/-) zebrafish at 5 mpf. In ovaries of homozygous (ar ^-/-), no follicles were observed and severe degeneration occurred (30%, n = 30). (P) lhcgr (luteinizing homone/choriogonadotropin receptor) expression in ovaries of wildtype (ar^+/+) and homozygous (ar^-/-) zebrafish at 4.5 mpf. (Q) foxl2 (forkhead box L2) expression in ovaries of wildtype (ar ^+/+) and homozygous (ar ^-/-) zebrafish at 4.5 mpf. (R) cyp11a1 (p450 side chain cleavage enzyme) expression in ovaries of wildtype (ar^+/+) and homozygous (ar ^-/-) zebrafish at 4.5 mpf. (S) kitlga expression in ovaries of wildtype (ar^+/+) and homozygous (ar ^-/-) zebrafish at 4.5 mpf injected with or without DHT (100 nM, 10μl for each). Dpf, days post fertilization; Mpf, months post fertilization.