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Fig. 2

bsx is expressed in all cell types of the PC except projection neurons. (A-F″) Double-fluorescent WISH of 3 dpf wild-type zebrafish embryos detects expression of bsx (magenta, A-F), PC type-specific marker genes (as indicated in green, A′-F′), and merged channels (A″-F″). All panels show single confocal planes (image depth z=1 µm; anterior is to the top). n≥2 for all. Maximum intensity projections of stacks are shown in Fig. S3. Scale bar: 20 µm.

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