IMAGE

Fig. 4

ID
ZDB-IMAGE-170703-17
Genes
Source
Figures for Nelson et al., 2017
Image
Figure Caption

Fig. 4

Loss of Ta and Tbx16 Leads to Downregulation of Endodermal Specifiers and Reduction of Endodermal Progenitors during Gastrulation

(A) qPCR analysis of mixl1, gata5, and sox32 at 50% epiboly (5.3 hpf) in single and double ta/tbx16 morphants. All genes are significantly downregulated in double morphants; ?p ? 5 × 10?2; Student?s t test. Data are represented as mean ± SEM. See also Figure S2.

(B) WISH analysis of sox32 in single and double morphants at germ ring stage (5.7 hpf). Arrowhead, YSL expression; ?, loss of endoderm expression.

(C) Microarray analysis at 90% epiboly (9 hpf) indicates downregulation of cxcl12a/b. Data are represented as mean ± SEM.

(D) Immunological and WISH analysis of a sox17:eGFP transgene and endogenous sox17 expression at 90% epiboly (9 hpf) in single and double morphants.

(E) Cell numbers identified by immunostaining and WISH in (D). Cell numbers are representative of at least 20 embryos per condition. ?p ? 1 × 10?8; ??p ? 1 × 10?20; Student?s t test. Data are represented as mean ± SEM.

See also Figure S3.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.