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Fig. 1
Expression patterns of crebbpa and crebbpb transcripts during zebrafish embryogenesis. Expression levels of crebbpa (red line) and crebbpb (blue line) were monitored by RT-qPCR at 2-cell, dome, shield, 6 ss, 14 ss, 16 ss, 20 ss, 24 hpf and 48 hpf. (B) Expression levels of crebbpa, crebbpb, myf5 and mlc2f in 10 anterior somites (rostral, red bars) and 10 posterior somites (caudal, black bars) of 20 ss embryos. Myf5 expression is specific of the nascent caudal somites while mlc2f, a specific marker of fast differentiating muscle fibres, is preferentially expressed in the rostral somites at this stage. All RT-qPCR data were normalized to EF1alpha housekeeping gene expression and data shown were from 3 independent experiments. (C) Whole-mount in situ hybridization against crebbpa at the indicated developmental stages. The line on the right at 18 ss indicates crebbpa enrichment in the trunk and caudal somites. *, PreSomitic Mesoderm; red arrow, lens; white arrowhead, otic vesicle; black arrow, somitic expression. (D) In situ hybridization against crebbpa and crebbpb at 18 ss (18 hpf) with rostral and caudal magnifications (red and black lines, respectively) of flat-mounted embryos. Black arrowhead, enrichment in caudal somites; red asterisk, enrichment in rostral somites. (E) Schematic summary of crebbpa and crebbpb expression at 18 ss. A dorsal view is represented (anterior to the top). (F) Transverse sections of 18 ss (upper panels) and 24 hpf (bottom panels) embryos from red (rostral) to black (caudal) lines noted in (D), showing crebbpa enrichment in the myotome at 18 ss (black arrowhead), in the lens (red arrow), in the retina (red arrowhead) and in the myotome (black arrow) at 24 hpf.