ZFIN Image: Su et al., 2014, Fig. 1

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Fig. 1

Gbx1 and Gbx2 function redundantly in cerebellum development. (A) Schematic of nonsense mutations identified in zebrafish gbx1 and gbx2 by TILLING. Both mutations are expected to prevent DNA binding by truncating the homeodomain. (B–I) Dorsal views at 22 hpf (B–E) or 6 dpf (F–I), anterior to the left. Genotypes are shown at the top. otx2 (blue), eng2b (red), and egr2b (blue) are expressed in midbrain, midbrain–hindbrain boundary (MHB), and rhombmere 3/5, respectively, in wildtype (WT), single and double mutants as shown. Tg(ptf1a:EGFP) (green) marks Purkinje neuron progenitors, Tg(olig2:DsRed2) (red) marks projection neurons, and anti-Vglut1/Slc17a7 (blue) marks cerebellar granule neuron axons. In gbx1^fh271;gbx2^fh253 double mutants the midbrain is expanded at the expense of r1 and no cerebellum forms.

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Acknowledgments

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Reprinted from Developmental Biology, 386(1), Su, C.Y., Kemp, H.A., and Moens, C.B., Cerebellar development in the absence of Gbx function in zebrafish, 181-90, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.