ZFIN Image: Zhang et al., 2012, Fig. S3

Image

Figure Caption

Fig. S3

No Effect of agrp MO in the mc4r-/- Background

(A-D) Whole mount in situ hybridization of gh (A-B) and pomca (C-D) in uninjected sa0149 wild type fish (A and C) or un-injected sa0149 mc4r -/- fish (B and D) at 4dpf. Scale bar: 100µm. (E) Relative expression levels of agrp, mc4r, gh, ghrh, pomca, pomcb were analyzed by Q-PCR. Offspring from sa0149 sibling wild type and sa0149 mc4r-/- matings were raised at standard condition. 30 uninjected embryos from each condition were divided into 3 groups and sacrificed at 4dpf for RNA extraction and cDNA synthesis. Wild type group was normalized to 100% and all gene expression was normalized to the house-keeping gene, ef1a. Results are expressed as mean + s.e.m., and statistical analysis was done by unpaired t-test (Not Significant for all genes). (F-I) Whole mount in situ hybridization of gh (F-G) and pomca (H-I) in mc4r -/- fish injected with 2.5ng standard control (F and H) or agrp morpholino oligonucleotides (G and I). Scale bar: 100µm.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Cell Metabolism, 15(2), Zhang, C., Forlano, P.M., and Cone, R.D., AgRP and POMC neurons are hypophysiotropic and coordinately regulate multiple endocrine axes in a larval teleost, 256-264, Copyright (2012) with permission from Elsevier. Full text @ Cell Metab.