ZFIN Image: Zhang et al., 2012, Fig. 3

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Figure Caption

Fig. 3 agrp Regulates Expression of Multiple Pituitary Hormones in Zebrafish

(A–F) Whole-mount in situ hybridization of gh (A and B) and pomca (C and D) in fish injected with 2.5 ng standard control (A and C) or agrp MO oligonucleotides (B and D). Scale bars: 100 µM. Relative expression levels of gh (growth hormone), ghrh (growth hormone-releasing hormone), sst1 (somatostatin 1), sst2 (somatostatin 2), pomca (pro-opiomelanocortin a), pit1 (pituitary-specific positive transcription factor 1), sml a (somatolactin a), sml b, tsh (thyroid-stimulating hormone), trh (thyrotropin-releasing hormone), crh (corticotrophin-releasing hormone), fshb (follicle-stimulating hormone), lhb (luteinizing hormone), igf1a (insulin-like growth factor 1a), and igf1b were analyzed by Q-PCR in 30–40 4 dpf fish injected with 2.5 ng standard control or agrp MO oligonucleotides (E and F). mRNA expression was normalized to ef1a mRNA. Results were expressed as mean + SEM, and statistical analysis was done by unpaired t test (p < 0.05; p < 0.001; NS, not significant). See also Figure S3

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Expression / Labeling details

Acknowledgments

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Reprinted from Cell Metabolism, 15(2), Zhang, C., Forlano, P.M., and Cone, R.D., AgRP and POMC neurons are hypophysiotropic and coordinately regulate multiple endocrine axes in a larval teleost, 256-264, Copyright (2012) with permission from Elsevier. Full text @ Cell Metab.