ZFIN Image: Bresciani et al., 2010, Fig. 3

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Figure Caption

Fig. 3 Expression of hematopoietic genes in nb/nbl morphants.

A–D. Flat mounted 10-ss embryos. Triple WISH were performed on std MO (A, C) and nb/nbl MO-injected embryos (B, D). The expression of myoD in somitic and pre-somitic mesoderm appeared unaltered in nb/nbl MO embryos (B, D). The expression of krox20 in the rombomeres 3 and 5 is unaffected in nb/nbl morphants (B, D). A–B. The expression of scl appears reduced in the posterior PLM (black arrowheads). C–D. gata1 expression is reduced in nb/nbl morphants, particularly in the posterior PLM (black arrowheads). E–N. Lateral view of 22–24 hpf std MO embryos (E, G, I, M) and nb/nbl morphants (F, H, L, N). In nb/nbl morphants at 22 hpf gata1 expression is reduced in the ICM (F); ikaros and βe1 globin are also downregulated at 24 hpf (H, L). The myeloid marker pu.1 is expressed at normal levels in nb/nbl MO-injected embryos (N).

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Expression / Labeling details

Acknowledgments

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