ZFIN Image: Nakamura et al., 2008, Fig. 6

Image

Figure Caption

Fig. 6 Nlz proteins act as repressors during zebrafish hindbrain development.A-H. Nlz proteins repress vhnf1 and gbx1 expression in rhombomere 4. Wild type embryos (A, C, E, G) or embryos injected with antisense morpholino oligos targeting Nlz1 and Nlz2 (Nlz^-; B, D, F, H) were assayed by whole mount in situ hybridization for expression of gbx1/vhnf1 (A, B: gbx1 red bracket, vhnf1 yellow bracket), gbx1 (C, D), pax2/vhnf1 (E, F; both detected in purple) and krox20/vhnf1 (G, H; krox20 detected in red, vhnf1detected in blue). White arrows in A and black brackets in E-H indicate gaps in gene expression. I-X. Removing vhnf1 function restores late gene expression to Nlz^-embryos. Wild type (I, M, Q, U), embryos injected with anti-Nlz MO (Nlz^-; J, N, R, V), vhnf1 mutant embryos (K, O, S, W) and vhnf1 mutant embryos injected with anti-Nlz MO (Nlz^-/vhnf1^-; L, P, T, X) were assayed by in situ hybridization for expression of krox20 /hoxb1a (I-P; krox20 detected in red, hoxb1a detected in blue) or krox20/fgf3 (Q-X; krox20 detected in red, fgf3 detected in blue) at the 14 somite stage (I-L; Q-T) or the tailbud stage (M-P; U-X). Embryos in I-X were dissected and flat-mounted such that only the hindbrain is shown. Anterior is to the top in A-H and to the left in I-X).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.